Thermo Fisher Q Exactive HF-X Hybrid Quadrupole Orbitrap MS System HRAM 40 Hz

Thermo Fisher Q Exactive HF-X Hybrid Quadrupole-Orbitrap MS System is a high-resolution, accurate-mass (HRAM) mass spectrometer designed for identification and quantitation in a single analysis. It combines an ultra-high-field Orbitrap mass analyzer with a quadrupole mass filter for precursor ion selection, supporting fast MS/MS acquisition up to 40 Hz using accelerated Higher-Energy Collisional Dissociation (aHCD). The system includes a High Capacity Transfer Tube (HCTT) with an electrodynamic ion funnel to improve sensitivity and ion transmission, along with advanced quadrupole precursor isolation technology and an HCD collision cell. Data acquisition supports Full Scan, SIM, PRM, DIA, and All Ion Fragmentation (AIF) modes, with polarity switching on the chromatographic timescale and spectrum multiplexing for up to 10 precursors/scan. At intake, the unit operated and passed basic function checks with no error codes, showing light to moderate cosmetic wear from prior use.

• Hybrid quadrupole-Orbitrap design for high-resolution, accurate-mass HRAM analysis
• Scan speed up to 40 Hz with accelerated Higher-Energy Collisional Dissociation (aHCD)
• High Capacity Transfer Tube HCTT with electrodynamic ion funnel to improve sensitivity and ion transmission
• Advanced Quadrupole Technology for improved precursor isolation and transmission
• Variable precursor ion isolation width selection from 0.4 Da to full mass range
• Orbitrap mass analyzer with nitrogen-filled C-Trap and straight multipole collision cell for HCD experiments
• Data acquisition modes include Full Scan, SIM, PRM, DIA, and All Ion Fragmentation AIF
• Spectrum multiplexing for detection of up to 10 precursor ions in the Orbitrap simultaneously
• Automatic Gain Control AGC support for consistent ion filling

• Resolution: 240,000 at m/z 200 (FWHM)
• Mass range: 50 to 6,000 m/z (up to 8,000 m/z with BioPharma option)
• Scan rate: up to 40 Hz at resolution setting of 7,500 at m/z 200
• Mass accuracy (internal): < 1 ppm RMS
• Mass accuracy (external): < 3 ppm RMS
• MS/MS sensitivity example: 100 fg reserpine on column S/N 150:1
• SIM sensitivity example: 50 fg reserpine on column S/N 150:1
• Dynamic range: > 5,000:1
• Cycle time: one full cycle in < 1 sec (positive and negative mode scans at resolution setting of 60,000)
• Multiplexity: up to 10 precursors/scan
• Analog inputs: one analog input (0-1 V)
• Analog inputs: one analog (0-10 V)
• Vacuum system final vacuum: < 1 x 10-9 mbar
• Power: 2 x 230 VAC +/- 10% single phase, 15 A, 50/60 Hz (instrument) with earth ground
• Power (data system): 120 or 230 VAC single phase with earth ground
• Operating environment: 15-26 C with relative humidity 40-70% with no condensation
• Operating altitude: up to 2000 m
• Dimensions (h x d x w): 950 x 830 x 910 mm
• System average power output: 2,800 W (~10,000 Btu/h) considering air conditioning needs
• Operating ion source: H-ESI II probe with dual desolvation zone technology
• Ion probe orientation: 60 degree interchangeable ion probe orientation
• H-ESI II compatible flow rates: <1 uL/min to 1 mL/min without splitting
• APCI/APPI compatible flow rates: 50 uL/min to 2 mL/min without splitting
• Nitrogen source gas minimum purity: 99% at 800 +/- 30 kPa (8.0 +/- 0.3 bar)
• HCD nitrogen gas recommended purity: 99.999% at 800 +/- 30 kPa (8 +/- 0.3 bar)
• Weight (system): 182 kg without forevacuum pumps

Imperial: 682.0 Pounds
Metric: 309.35 Kilograms

Imperial: 62.4 in x 424.8 in x 54.0 in
Metric: 158.5 cm x 1078.99 cm x 137.16 cm